The goal of posDemux is to provide tools for tools for handling reads with combinatorial barcodes, and multiple adapter regions. This includes utilities for demultiplexing, sequence segmenting and filtering. The package is intended to work with single-cell RNA-seq data with multiple barcoding (e.g. SPLiT-seq and PETRI-seq).
Installation
You can install the development version of posDemux from GitHub with:
# install.packages("devtools")
devtools::install_github("yaccos/posDemux")Usage
Please see the package vignettes.